What is needed for DNA replication lagging strand?

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Cdc24 and Dna2 form a complex in vivo. (A) Dna2-Myc co-purifies with Cdc24-HA. Native protein extracts were prepared from cdc24-HA dna2-Myc ura4-D18 leu1-32 h− (lanes 1 and 3) and dna2-Myc ura4-D18 leu1-32 h+ (lanes 2 and 4) cells growing exponentially in EMM medium supplemented with leucine and uracil, and Cdc24-HA immunoprecipitated with anti-HA antibody. Cell extracts (lanes 1 and 2) and immunoprecipitates (lanes 3 and 4) were analysed by immunoblotting with anti-HA (upper panel) and anti-Myc (lower panel) antibodies. (B) Cdc24-Myc co-purifies with Dna2-HA. The same procedures were performed with dna2-HA cdc24-Myc ura4-D18 leu1-32 h− (lanes 1 and 3) and cdc24-Myc ura4-D18 leu1-32 h+ (lanes 2 and 4) cells, but in this case, Dna2-HA was immunoprecipitated with anti-HA antibody. Cell extracts (lanes 1 and 2) and immunoprecipitates (lanes 3 and 4) were then analysed by immunoblotting with anti-HA (upper panel) and anti-Myc (lower panel) antibodies. Molecular weight markers (kD) are shown to the left of each panel. Note that multiple forms of Dna2 can be detected by immunoblotting. Whether these represent post-translationally modified isoforms of the protein, or merely degradation products, is not known.

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