Issues with DNA Fingerprinting in Forensic Lab: A Review Institute of Molecular Biology & Biotechnology, Bahaudin Zakariya University, Multan-60800, Pakistan *Corresponding Author: Rana Khalid Iqbal, Institute of Molecular Biology & Biotechnology, Bahaudin Zakariya University, Multan-60800, Pakistan. Received: March 05, 2019; Published: April 03, 2019 Successful interpretation of DNA is influenced by the value of crime scene evidence and the availability of suitable references samples. Errors and uncertain results are the hidden sides of DNA fingerprinting. Efforts are underway to improve the results in this field. The DNA is chosen here because it is extensively practiced and act as a DNA ID card whereas DNA finger-printing is an optimistic technique for forensic scientists. The target of this paper is to develop a professional and scientific view on the issues with DNA profiling in a forensic lab. In this paper a short outlook about DNA profiling objectives and techniques discussed but the main aim of this paper is issued about DNA fingerprinting. It is an interesting technique to investigate someone because DNA finger-printing also identifies the non-coding regions of the genome which makes discrimination among individuals of a species. Keywords: DNA profiling; RFLP; VNTR’s; DNA typing; PCR; Genomic finger-printing Abbreviation: DNA (Deoxyribose nucleic acid), PCR (Polymerase chain reaction), VNTR’s (Variable number tandem repeats), RFLP (Restriction fragment length polymorphism), COIDS (Combined index DNA system). An exclusive type of fingerprinting is known as “DNA fingerprinting”, it rests the same in all body parts and there is no method to modify it. DNA of every person is 99.9% identical but there is a 0.1% differences among individuals. The DNA sequence is just like fingerprinting. There are so many bases which are time-consuming but now scientist introduced some techniques which quicken the identification process. This (DNA fingerprinting) technique is like a bar-code of an individual's DNA [1]. DNA is found in every cell of an individual. DNA is tightly bound with histone protein, it is present in each chromosome [2]. It had a tremendous impact in forensic genetics. Forensic science is an intersection between crime and science. Its help to police inspect all the serious case and also an important tool for court outcomes. The progress of forensic science services clearly depends on police investigators understanding the scope, importance, and limitation of forensic science. It has perpetuated challenges for individual's and the court also accept as pivot evidence [3]. DNA typing played a supportive role and was the last resort when other methodologies failed. It provides an impressive approach in victim identification both at small scale and large scale disaster. It became a gold standard for victim identification. The U.S National Research Council of the National Laboratories issued a major report on forensic science in February 2009 “With the exception of nuclear DNA analysis, no forensic method thoroughly shown to have the capacity to consistently, and with a high degree of certainty, demonstrate a connection between evidence and a specific individual or source”. VNTR’s are variable number tandem repeats. These are noncoding regions and don’t have any unravel information about the genome. Genetic material is absent in these regions. So these regions are helpful in identifying a person. Potential samples for DNA investigation This technique was discovered by Sir Alec Jeffery in 1984 at Leicester University in the UK. He was a geneticist and work on genes. He was focusing on the methods to determine the paternity and colonization disputes by signifying the genetic links between persons. The first person who was arrested by DNA investigation Colin Pitchfork. He raped and then murdered to two girls Lynda and Dawn in1983 and 1986 respectively. Semen was collected by investigators and analyzed in a forensic lab to clear the whole story. When DNA pieces of evidence prove that Colin was a criminal then he confessed his crime and arrested [5]. The era of the 1990s is the golden period of new technologies and discoveries. This era proves valuable for forensic scientists. This technology used to check the whether the sera of the same person or not [6] Use of DNA finger-printing Goals Techniques Critical challenges Privacy issues Lack of expertise Low template DNA Touch-DNA Ecological impacts Fake DNA marks Instrumental troubles Hacking DNA proved an innocence as well as a guilty person. Errors can be made when samples are collected. DNA evidence is completely conclusive if the samples have not been contaminated. The lack of suitable experiments leads to the wrong perception, but advancement in molecular genetics avoid the types of contamination. Allowing the trained person to educate the public about DNA reliability. Allowing new technologies which show acceptance towards common people, make standardized tools and technologies of DNA typing. People think that there is a chance to misuse the data from DNA database by enforcing agencies. COIDS used DNA data only for identification but to proof identity not the whole genome required only core 13 loci is enough for identification and after keeping core 13 loci the remaining DNA should be carefully dispose-off if it is not required and one more thing is that 13 loci enough for identification it cannot give complete medical information of an individual. DNA evidence is not a reliable tool for criminal, experts have warned because there are man-made mistakes occur which lead to the wrong consequences. A study has found that interpretation of samples can be highly subjective and prone to error. The incredibly small amount of DNA in samples and pressure to gain a conviction can lead to bias results. Although the margin to biological challenges is near to nothing, the room for human mishandling always here. Poor laboratory practices can lead to false results. There is a possibility that DNA at crime scene replaced by another person, who was not a criminal actually. Forensic DNA typing had a tremendous positive impact in the criminal justice system but its reliability should not be taken granted. DNA of each and every person is a God’s signature which discriminate every individual but our carelessness make it fragile which arise questions at its reliability.
Citation: Anam Hameed Sahiba Sahar, Javeria Bibi and Rana Khalid Iqbal. (2019). Issues with DNA Fingerprinting in Forensic Lab: A Review. Journal of Medical Research and Case Reports 1(1). Copyright: © 2019 Rana Khalid Iqbal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |